Transcriptomic analysis in human 3D skin model injected with resorbable hyaluronic acid fillers reveals foreign body response [Perfectha]

To unravel the molecular events caused by exposure to dermal fillers in a multi-cell type human 3D skin model and to link these events to the characteristics of the dermal fillers. The human Phenion® Full-Thickness Skin Model was injection with four commercial fillers from the Perfectha® product line differing in particulate size. This was done in triplicate, thus 3 biological replicates were obtained. For each biological triplicate 2 control samples were included (i.e. technical replicates). In total this would account for 18 samples. However for each of the controls only 1 technical replicate (with the best quality) was selected and the Perfectha® Deep replicate 1 sample was excluded because it did not pass the quality criteria.