GUIDE-Seq for TRAC sgRNAs
收藏NIAID Data Ecosystem2026-03-12 收录
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资源简介：
GUIDE-Seq was performed of two CRISPR-Cas9 single guide RNAs used to target an Streptococcus pyogenes Cas9 nuclease (SpCas9) to the TRAC gene locus. The two sgRNAs differ in one position of their respective target-specific spacer sequence (TRAC Wiebking 2020: 5-GAGAATCAAAATCGGTGAAT-3; TRAC Charite: 5- GGGAATCAAAATCGGTGAAT-3). The study was performed to compare the location and the amount of putative off-targets sites of the respective sgRNAs in SpCas9-overexpressing HEK293 cells.
创建时间：
2021-02-11
