Oligonucleotide primer pairs used for PCR measurements.
收藏NIAID Data Ecosystem2026-03-07 收录
数据链接：
官方服务：
资源简介：
m … mouse.
应用场景：
创建时间：
2013-02-13
相关数据集
Additional file 3: Table S2. of Combining RNA-seq and proteomic profiling to identify seminal fluid proteins in the migratory grasshopper Melanoplus sanguinipes (F)
Primers used in RT-PCR and PCR conditions. PCR was performed with the following thermal conditions 60 s at 95 °C, 50 s at 94 °C, 30 s at 62 °C, 3 cycles /°C declining over a range at 1 °C intervals of
DataCite Commons2024-12-17 更新70
Characteristics of the primers used for SYBR green Q-PCR assays.
*Letters F and R after the primer name indicate forward and reverse orientation, respectively. †Theoretical melting temperature (Tm) calculated using the MWG Oligo Property Scan (MOPS).
NIAID Data Ecosystem60
C t value for PCR reagent stored at 37°C for different days.
aCt: threshold cycle;values<35 are considered to be positive. Each Ct value is the mean result of the three values obtained each time point. bCV: coefficients of variations.
NIAID Data Ecosystem50
Additional file 10: of Expanding the biodiversity of Oenococcus oeni through comparative genomics of apple cider and kombucha strains
Table S3. Primer list. The sequence surrounding NNN-islands in the CRBO_1381 assembly scaffold was entered into Primer3 with default settings (GC clamp = 1) to find suitable primersets for PCR product
Mendeley Data2024-06-27 更新70
Primers used and PCR amplicons generated in this study.
*The restriction endonuclease (R.E.) site included in each primer is underlined and the corresponding R.E. indicated in the adjacent column. #The amplicons generated with a given set of PCR primers ar
NIAID Data Ecosystem60
