We describe a robust linear amplification-mediated high-throughput genome-wide translocation sequencing (HTGTS) method that identifies endogenous or ectopic "prey" DNA double-stranded breaks (DSBs) ac

NIAID Data Ecosystem70

Chromomal translocations induced by CRISPR-Cas9 persist in expanded T cells

基因编辑安全性

T细胞治疗

Chromosomal translocations cause various lymphoid cancers and are prevalent in CRISPR-Cas9-meidated gene editing. However, the fate of gene editing-induced translocations remains elusive in vivo. Here

NIAID Data Ecosystem40

Optimizing genome editing strategy by primer-extension-mediated sequencing

CRISPR脱靶检测

基因组优化

High specificity of e\ngineered nucleases ensures precise genome editing. Couple methods were developed to identify off-target sites of CRISPR/Cas9, but hardly any high-throughput sequencing method ca

NIAID Data Ecosystem70

Improving the sensitivity of in vivo CRISPR off-target detection through DNA repair inhibition. Homo sapiens and Mus musculus

CRISPR脱靶检测

基因编辑安全性

Improving the sensitivity of in vivo CRISPR off-target detection through DNA repair inhibition

NIAID Data Ecosystem50