Ori-Finder 3

Ori-Finder 3, for the computational prediction of replication origins in S. cerevisiae at the genome-wide level based solely on DNA sequences. The ARS exhibiting both an AT-rich stretch and ARS consensus sequence element can be predicted at the single-nucleotide level. For the identified ARSs in the S. cerevisiae reference genome, 83 and 60% of the top 100 and top 300 predictions matched the known ARS records, respectively. Based on Ori-Finder 3, we subsequently built a database of the predicted ARSs identified in more than a hundred S. cerevisiae genomes. Consequently, we developed a user-friendly web server including the ARS prediction pipeline and the predicted ARSs database.Ori-Finder 3是一款仅依托DNA序列，可在全基因组水平上对酿酒酵母（Saccharomyces cerevisiae）复制起点开展计算预测的工具。同时具备AT富集区段与自主复制序列（Autonomously Replicating Sequence，ARS）共识序列元件的ARS，可在单核苷酸水平上完成预测。针对酿酒酵母参考基因组中已鉴定的ARS，其预测排名前100和前300的结果中，分别有83%和60%与已知ARS记录相匹配。基于Ori-Finder 3，我们进一步构建了覆盖百余株酿酒酵母基因组的预测ARS数据库。据此，我们开发了一款界面友好的Web服务器，集成了ARS预测流程与上述预测ARS数据库。

搜集汇总

背景与挑战

背景概述

Ori-Finder 3是一个计算工具，专门用于基于DNA序列在全基因组水平预测酿酒酵母的复制起点，能够识别富含AT区域和ARS共有序列元件，并在单核苷酸精度进行预测。该工具还提供了预测ARS数据库和用户友好的网络服务器，以支持相关研究。