RB-loss determines selective resistance and novel vulnerabilities in breast cancer modelsRB loss

The retinoblastoma tumor suppressor (RB1) plays a critical role in coordinating multiple pathways that impact on tumor initiation, disease progression, and therapeutic responses. Here we interrogated the effect of cIAP inhibitor, birinapant in two different isogenic ER+ breast cancer models. Using MCF7-WT and MCF7-RB-del cell lines we investigated the differential gene expression pattern that link the effect of CIAP inhibition and RB dependency . Overall design: MCF7-WT and MCF7-RB-del cells were treated with birinapant at a concentration of 500 nM for 48 hours. RNA was extracted using Quiagen Rneasy plus kit.