Microarray analysis of <i>ATF2^+/+TyrCre⁺Nras⁺Ink4a^−/−</i> and <i>ATF2^−/−TyrCre⁺Nras⁺Ink4a^−/−</i>melanocytes and confirmation by qPCR.

Primary melanocytes were isolated and inactivation of ATF2+ induction of N-Ras expression was performed in culture (see Materials and Methods for details). RNA prepared from the cells was used for array analysis. Data shown represents 2 biological and 2 technical replicates for the array studies and 3 replicates for the qPCR analyses (see Materials and Methods for details).