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Primer sequences used for qRT-PCR validation of the microarray data.

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数据链接：

https://figshare.com/articles/dataset/_Primer_sequences_used_for_qRT_PCR_validation_of_the_microarray_data_/1067578 数据链接链接失效反馈

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资源简介：

Primer sequences used for qRT-PCR validation of the microarray data.

应用场景：

创建时间：

2014-06-23

T细胞活化通路

基因表达验证

Gene expression levels of target genes were assessed by real time PCR in PBMCs from 16 new healthy controls after stimulation with IL10, IL4 or CTLA4 (stimulus) for 12 to 24h. Results are described as

Figshare2015-12-02 更新40

Quantitative PCR (RT-qPCR) validation of RNA-seq using liver samples from each of the four comparisons, illustrated in Fig 1.

基因表达验证

RNA-seq验证

Quantitative PCR (RT-qPCR) validation of RNA-seq using liver samples from each of the four comparisons, illustrated in Fig 1.

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Validation of SAGE-type library comparison results and analysis of expression levels of genes encoding proteins involved in infection thread formation by quantitative real-time polymerase chain reaction (qPCR).

根瘤菌共生

基因表达验证

Values for qPCR results represent means of three biological replicates. *Contigs were originally classified based on 454 sequencing results. The tendency (nodule-enhanced or root-enhanced expression w

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Ct values for a skeletal muscle cDNA control demonstrate no plate-to-plate variability.

qPCR实验质量控制

基因表达验证

For each qPCR plate, a skeletal muscle cDNA control sample was included to assess plate-to-plate variability for every primer pair. For the Tissue Panel experiment Ct values represent three skeletal m

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Top 20 overexpressed genes (>10 fold) in both CS-1/ER and CS-1/PR resistant cell lines.

细菌耐药性机制

基因表达验证

*Genes in bold were chosen for real-time RT-PCR validation.

Figshare2015-12-02 更新20