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Primer sequences used for differentially expressed genes used for qPCR validation.

Figshare2024-03-13 更新2026-04-28 收录

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https://figshare.com/articles/dataset/Primer_sequences_used_for_differentially_expressed_genes_used_for_qPCR_validation_/25402086 数据链接链接失效反馈

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Primer sequences used for differentially expressed genes used for qPCR validation.

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创建时间：

2024-03-13

小胶质细胞对伯氏疏螺旋体转录反应

基因表达验证

Transcript RNA collected from microglia cultured in the presence of B. burgdorferi (Bb) was compared to that of RNA collected from microglia (μG) cultured in medium alone. RT-PCR reactions were run in

NIAID Data Ecosystem60

Quantitative PCR (RT-qPCR) validation of RNA-seq using liver samples from each of the four comparisons, illustrated in Fig 1.

基因表达验证

RNA-seq验证

Quantitative PCR (RT-qPCR) validation of RNA-seq using liver samples from each of the four comparisons, illustrated in Fig 1.

NIAID Data Ecosystem20

Log2 fold changes of the four specifically amplified genes for validation resulting from the MACE analysis and RT-qPCR (calculated using REST with correction of amplification efficiencies).

基因表达验证

实时定量PCR数据分析

SE = standard error.Log2 fold changes of the four specifically amplified genes for validation resulting from the MACE analysis and RT-qPCR (calculated using REST with correction of amplification effic

Figshare2015-12-03 更新20

Ct values for a skeletal muscle cDNA control demonstrate no plate-to-plate variability.

qPCR实验质量控制

基因表达验证

For each qPCR plate, a skeletal muscle cDNA control sample was included to assess plate-to-plate variability for every primer pair. For the Tissue Panel experiment Ct values represent three skeletal m

NIAID Data Ecosystem30

Validation of SAGE-type library comparison results and analysis of expression levels of genes encoding proteins involved in infection thread formation by quantitative real-time polymerase chain reaction (qPCR).

根瘤菌共生

基因表达验证

Values for qPCR results represent means of three biological replicates. *Contigs were originally classified based on 454 sequencing results. The tendency (nodule-enhanced or root-enhanced expression w

NIAID Data Ecosystem20

Primer sequences used for differentially expressed genes used for qPCR validation.

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