1Amplification was performed in a GeneAmp 9700 PCR System (Applied Biosystems) by a 10 min incubation at 95°C, followed by 45 cycles of 301 s. at 95°C, 45 s. at 60°C and 60 s. at 72°C. The program was

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Evidence supporting the MICU1 occlusion mechanism and against the potentiation model in the mitochondrial calcium uniporter complex. Tsai et al.

线粒体钙信号

蛋白质合成机制

Unprocessed raw Western-blot or BN-PAGE images used in the figures in Tsai et al., PNAS, 2023.

NIAID Data Ecosystem50

Liquid-based method for isolation of total DNA.

DNA提取技术

分子生物学实验方法

Liquid-based method for isolation of total DNA.

Figshare2020-06-01 更新30

The conserved aspartate ring of MCU mediates MICU1 binding and regulation in the mitochondrial calcium uniporter complex

线粒体钙信号

蛋白质相互作用调控

The mitochondrial calcium uniporter is a Ca2+ channel that regulates intracellular Ca2+ signaling, oxidative phosphorylation, and apoptosis. It contains the pore-forming MCU protein, which possesses a

DataONE2020-06-24 更新60

Supplementary material 8 from: Marquina D, Roslin T, Łukasik P, Ronquist F (2022) Evaluation of non-destructive DNA extraction protocols for insect metabarcoding: gentler and shorter is better. Metabarcoding and Metagenomics 6: e78871. https://doi.org/10.3897/mbmg.6.78871

生物样本制备

分子生物学实验方法

Lysis buffers and purification protocols

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