Primers used in RT-PCR and PCR conditions. PCR was performed with the following thermal conditions 60 s at 95 °C, 50 s at 94 °C, 30 s at 62 °C, 3 cycles /°C declining over a range at 1 °C intervals of
*The restriction endonuclease (R.E.) site included in each primer is underlined and the corresponding R.E. indicated in the adjacent column. #The amplicons generated with a given set of PCR primers ar
aCt: threshold cycle;values<35 are considered to be positive. Each Ct value is the mean result of the three values obtained each time point. bCV: coefficients of variations.
Table S3. Primer list. The sequence surrounding NNN-islands in the CRBO_1381 assembly scaffold was entered into Primer3 with default settings (GC clamp = 1) to find suitable primersets for PCR product