Transcriptomic analysis of liver mRNA from liver-specific Pck1-knockout mice fed with chow diet or NASH diet

To investigate the role of hepatic Pck1 in diet-induced nonalcoholic steatohepatitis (NASH), liver-specific Pck1-knockout mice were developed with Alb-Cre. Wild-type (WT) and Pck1-cKO mice were fed a Chow diet (Research Diets, D12450J: 10% Kcal fat, with tap water) or NASH-inducing diet (Research Diets, D12492: 60% Kcal fat, with drinking water containig 23.1 g/L fructose and 18.9 g/L glucose) for 24 weeks. RNA was extracted from the livers of mice from all treatment groups and used for RNA seqencing. RNA-seq data demonstrated that gluconeogenic enzyme PCK1 deficiency played an important role in the development of NASH. Use RNA-sequencing to investigate the role of Pck1 in diet-induced NASH on a transcriptomic level, by comparing the livers of WT and Pck1-cKO mice fed a Chow or NASH diet for 24 weeks using Illumina technology.为探究肝脏Pck1在饮食诱导的非酒精性脂肪性肝炎（nonalcoholic steatohepatitis, NASH）中的作用，本研究通过Alb-Cre系统构建了肝脏特异性Pck1基因敲除小鼠。将野生型（Wild-type, WT）与肝脏特异性Pck1基因敲除（Pck1-cKO）小鼠分别饲喂普通饲料（Research Diets公司货号D12450J：热量占比10%的脂肪饲料，饮用自来水）或非酒精性脂肪性肝炎造模饲料（Research Diets公司货号D12492：热量占比60%的脂肪饲料，饮用添加23.1 g/L果糖与18.9 g/L葡萄糖的饮用水），持续24周。采集所有处理组小鼠的肝脏组织并提取总RNA，用于RNA测序。RNA测序结果表明，糖异生关键酶PCK1的缺失在NASH的发生发展中发挥了重要作用。本数据集采用Illumina测序技术，通过对比饲喂普通饲料或NASH造模饲料24周的野生型与Pck1-cKO小鼠的肝脏转录组，从转录组层面探究Pck1在饮食诱导NASH中的作用。