Gene expression levels of target genes were assessed by real time PCR in PBMCs from 16 new healthy controls after stimulation with IL10, IL4 or CTLA4 (stimulus) for 12 to 24h. Results are described as
SE = standard error.Log2 fold changes of the four specifically amplified genes for validation resulting from the MACE analysis and RT-qPCR (calculated using REST with correction of amplification effic
For each qPCR plate, a skeletal muscle cDNA control sample was included to assess plate-to-plate variability for every primer pair. For the Tissue Panel experiment Ct values represent three skeletal m
Values for qPCR results represent means of three biological replicates. *Contigs were originally classified based on 454 sequencing results. The tendency (nodule-enhanced or root-enhanced expression w
