RNA-seq of postnatal day 0 (P0) wild-type, Fezf2-/- cortices, and Fezf2-/-;Fezf2-EnR cortices

The goal of the study was to compare gene expression of P0 wild-type, P0 Fezf2-/- cortices, and Fezf2-/-; Fezf2-EnR cortices. Total RNAs were isolated from P0 cortices dissected from wild-type (n=3), Fezf2-/- mice (n=4), and Fezf2-/-; Fezf2-EnR cortices (n=2), following Qiagen RNAeasy kit instruction.Sequence libraries were made following Illumina RNA PolyA library preparation guide.The libaries were pair-end sequenced (150nt per end). Differentially expressed genes were identified by DESEQ. Total RNAs were isolated from P0 cortices, and sequenced on Illumina Novaseq4本研究旨在比较P0阶段野生型皮层、Fezf2基因敲除（Fezf2-/-）皮层以及Fezf2-/-; Fezf2-EnR皮层的基因表达谱。按照Qiagen RNAeasy试剂盒 (Qiagen RNAeasy kit) 的操作说明，分别从野生型小鼠（n=3）、Fezf2-/-小鼠（n=4）及Fezf2-/-; Fezf2-EnR皮层样本（n=2）的P0小鼠皮层中分离总RNA。测序文库的构建遵循Illumina RNA PolyA文库制备指南 (Illumina RNA PolyA library preparation guide)。随后采用双端测序模式（每端读长150nt）对文库进行测序。差异表达基因通过DESEQ进行鉴定。本实验同时从P0小鼠皮层中提取总RNA，并在Illumina NovaSeq 4测序平台上完成测序。