Transcript RNA collected from microglia cultured in the presence of B. burgdorferi (Bb) was compared to that of RNA collected from microglia (μG) cultured in medium alone. RT-PCR reactions were run in
For each qPCR plate, a skeletal muscle cDNA control sample was included to assess plate-to-plate variability for every primer pair. For the Tissue Panel experiment Ct values represent three skeletal m