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CRISPRi-mediated functional analysis of lung disease-associated loci at non-coding regions [RNA-seq]

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NIAID Data Ecosystem2026-03-14 收录
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RNA-seq was performed to identify genes affected by CRISPRi (dCas9-KRAB) with sgRNA (sgTGFB1_B9D2) targeting a region that contains rs1800469 in an unbiased genome-wide fashion using H441 lung papillary adenocarcinoma cells. Overall design: Expression of genes in H441 cells stably expressing dCas9-KRAB with the sgRNA was compared with those stably expressing dCas9-KRAB without the sgRNA.

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2023-01-11
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