Estimated tree using data from Mendlová and Šimková (2014)

Our estimated tree generated using data from Mendlová and Šimková (2014). The tree is based on two partial ribosomal RNA genes (SSU and LSU) and the entire ITS1 nuclear gene region for 25 species in the genus Cichlidogyrus and 3 species in the genus Scutogyrus. The accession numbers were taken from Mendlová et al. (2012) . The tree was estimated using BEAST v2.1.3 (Bouckaert et al., 2014) on the constrained topology from Mendlová and Šimková (2014) . We used a GTR + Γ model of nucleotide evolution, separate partitions for each gene, a relaxed log-normal clock, and birth-death priors. Further information and full references can be found in the supplementary material of our paper.