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Comparison of one-step and nested PCR protocol features.

Figshare2020-07-23 更新2026-04-28 收录

PCR技术比较

分子生物学实验方法

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https://figshare.com/articles/dataset/Comparison_of_one-step_and_nested_PCR_protocol_features_/12701081 数据链接链接失效反馈

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资源简介：

Comparison of one-step and nested PCR protocol features.

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创建时间：

2020-07-23

PCR扩增

分子生物学实验方法

1Amplification was performed in a GeneAmp 9700 PCR System (Applied Biosystems) by a 10 min incubation at 95°C, followed by 45 cycles of 301 s. at 95°C, 45 s. at 60°C and 60 s. at 72°C. The program was

NIAID Data Ecosystem80

Liquid-based method for isolation of total DNA.

DNA提取技术

分子生物学实验方法

Liquid-based method for isolation of total DNA.

Figshare2020-06-01 更新30

Concordance of genotype calls between Kapa 2GFast HotStart DNA polymerase and QIAGEN Multiplex PCR Kit.

基因分型技术验证

PCR技术比较

Concordance of genotype calls between Kapa 2GFast HotStart DNA polymerase and QIAGEN Multiplex PCR Kit.

NIAID Data Ecosystem30

Supplementary material 8 from: Marquina D, Roslin T, Łukasik P, Ronquist F (2022) Evaluation of non-destructive DNA extraction protocols for insect metabarcoding: gentler and shorter is better. Metabarcoding and Metagenomics 6: e78871. https://doi.org/10.3897/mbmg.6.78871

生物样本制备

分子生物学实验方法

Lysis buffers and purification protocols

NIAID Data Ecosystem40

Additional file 10 of The complete mitochondrial genome of the citrus red mite Panonychus citri (Acari: Tetranychidae): high genome rearrangement and extremely truncated tRNAs

线粒体测序

分子生物学实验方法

Additional file 10: Primer sequences and PCR conditions used to amplify the mitochondrial genome of Panonychus citri. (XLS 24 KB)

Figshare2010-10-23 更新30

Comparison of one-step and nested PCR protocol features.

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