Inter-day experimental variation without and with ESM. Note: SM8: surgically removed, formalin-fixed, paraffin-embedded (FFPE) sample 8. ISM D (−12/−14) contains ACTB IS 10−12M/target gene IS 10−14M t
For each qPCR plate, a skeletal muscle cDNA control sample was included to assess plate-to-plate variability for every primer pair. For the Tissue Panel experiment Ct values represent three skeletal m
Comparison of the mean Ct value of the internal amplification control in no-template controls and blood cultures tested negative for Leptospira sp. DNA.
