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Validation of microarray results by real-time quantitative RT-PCR (qRT-PCR).

NIAID Data Ecosystem2026-03-07 收录

数据链接：

https://figshare.com/articles/dataset/_Validation_of_microarray_results_by_real_time_quantitative_RT_PCR_qRT_PCR_/355789 数据链接链接失效反馈

官方服务：

资源简介：

Fold changes with P<0.05 are shown in bold.

应用场景：

创建时间：

2012-02-08

小胶质细胞对伯氏疏螺旋体转录反应

基因表达验证

Transcript RNA collected from microglia cultured in the presence of B. burgdorferi (Bb) was compared to that of RNA collected from microglia (μG) cultured in medium alone. RT-PCR reactions were run in

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Ct values for a skeletal muscle cDNA control demonstrate no plate-to-plate variability.

qPCR实验质量控制

基因表达验证

For each qPCR plate, a skeletal muscle cDNA control sample was included to assess plate-to-plate variability for every primer pair. For the Tissue Panel experiment Ct values represent three skeletal m

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Quantitative PCR (RT-qPCR) validation of RNA-seq using liver samples from each of the four comparisons, illustrated in Fig 1.

基因表达验证

RNA-seq验证

Quantitative PCR (RT-qPCR) validation of RNA-seq using liver samples from each of the four comparisons, illustrated in Fig 1.

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Additional file 12: of Insights from transcriptome profiling on the non-photosynthetic and stomatal signaling response of maize carbonic anhydrase mutants to low CO2

基因表达验证

玉米低二氧化碳响应

Primer sequences and IDs of genes selected for qRT-PCR validation. (XLSX 9 kb)

Figshare2019-02-16 更新40

RNAseq gene expression validation using by qPCR.