Catalytic efficiency (Vmax/Km) at 400 µM ATP.

Vmax/Km values were calculated from the linear part of v vs. [peptide] plots generated on a custom PamChip® peptide array. The fold change for each peptide with respect to JH1-JH2 WT and the percentage of the sum of all Vmax/Km for each protein are also indicated.本研究通过定制化PamChip®肽芯片生成的反应速率v与肽浓度[肽]的关系曲线图的线性区段，计算得到了Vmax/Km（最大反应速率/米氏常数）值。同时，还标注了每种肽相对于JH1-JH2野生型（JH1-JH2 WT）的倍数变化，以及每种蛋白的全部Vmax/Km值之和的占比。