Biochemical Reconstitution of UV-induced Mutational Processes

This dataset is associated with the paper entitled "Biochemical Reconstitution of UV-induced Mutational Processes", which is published in Nucleic Acid Research in 2019."Fastq.zip" contains all fastaq raw data files that have been used in the paper. Each fastaq file name contains NGS ID (18G, 17E etc) and barcode # (BCxx). These identifications match with Table S2 of the paper, which summarizes experimental conditions. "NGS Protocol 3.4.docx" is our laboratory protocol for basic analysis of our NGS data. This protocol is designed for new student to setup software and compute mutation frequency from fastaq data. Protocol uses three Excel template files with macro, which are also included in this dataset. The protocol and macros are designed for the specific data analysis, not for general use. Macro works only for specific template sequences used in the paper.本数据集配套发表于2019年《核酸研究》（Nucleic Acid Research）、题为《紫外线诱导突变过程的生化重构》（Biochemical Reconstitution of UV-induced Mutational Processes）的论文。"Fastq.zip"压缩包内收录了该论文中使用的全部FASTQ（Fastq）原始数据文件，每份FASTQ文件的文件名均包含NGS（Next-Generation Sequencing）标识（如18G、17E等）与条形码编号（BCxx）。此类标识与论文的补充表S2完全匹配，该表汇总了本次实验的各项条件。"NGS Protocol 3.4.docx"为本实验室用于NGS数据基础分析的操作规程，专为新生配置分析软件、从FASTQ数据中计算突变频率而设计。该规程需依托三份携带宏指令的Excel模板文件运行，此类模板文件亦收录于本数据集内。本规程与配套宏指令仅针对本次研究的特定数据分析场景设计，不具备通用性，且仅可与论文中使用的特定模板序列配合运行。