20240305 Gray Figure S1 data

<b>FIG S1 </b><b>Thiocyanate does not affect the minimal inhibitory concentration of nickel for </b><b><i>Escherichia coli</i></b><b>. </b><i>E. coli</i> strain MG1655 was grown overnight at 37°C with shaking in MOPS minimal medium containing 0.2% glucose, then normalized to A₆₀₀ = 1 and rinsed twice with sterile MOPS minimal medium containing 0.2% glucose but lacking micronutrients. The resulting cell suspensions were diluted 1:40 into MOPS minimal media containing 0.2% glucose without micronutrients, supplemented with the indicated concentrations of NiSO₄ and/or NaSCN. Growth curves were performed in clear 96-well plates in a Tecan Spark plate reader, incubating at 37°C with shaking and measuring A₆₀₀ at 30-minute intervals for 22 hours (n=3 experimental replicates, with 1-3 technical replicates per experiment; error bars of one standard deviation). Dashed lines indicate A₆₀₀ = 0.1 and t = 18 h.图S1 硫氰酸盐不影响镍对大肠杆菌（Escherichia coli）的最低抑菌浓度。大肠杆菌菌株MG1655于37℃下振荡培养过夜，培养基为含0.2%葡萄糖的MOPS基础培养基；随后将菌液归一化至A₆₀₀=1，并用不含微量营养成分、含0.2%葡萄糖的无菌MOPS基础培养基洗涤两次。将所得细胞悬液以1:40的比例稀释至不含微量营养成分、含0.2%葡萄糖的MOPS基础培养基中，补加指定浓度的硫酸镍（NiSO₄）和/或硫氰酸钠（NaSCN）。生长曲线检测在透明96孔板中进行，采用Tecan Spark酶标仪于37℃振荡孵育，每隔30分钟测定一次A₆₀₀，持续时长22小时（共3次生物学重复，每次实验设置1-3次技术重复；误差棒代表一个标准差）。图中虚线分别对应A₆₀₀=0.1与t=18小时。