Assessing impact of MALDI mass spectroscopy on reducing directed antibiotic coverage time for Gram-negative organisms

The objective of this study was to assess whether use of matrix assisted laser desorption ionization-time of flight (MALDI-TOF), through improvements in identification time, reduces time to directed antibiotic coverage. We therefore conducted a retrospective review of 377 blood cultures from hospitalized patients with gram negative bacteremia that underwent testing by MALDI-TOF compared to standard identification methods (VITEK 2) for blood cultures from January 2016 to December 2017. We found that MALDI significantly reduced time between blood culture collection to reach pathogen identification and was associated with a significantly reduced time to initiate more specific therapy, with a mean difference of 16.37 hours, 95% CI 10.05 to 22.69 (mean time 50.34 hours (+/- 21.21) vs VITEK: 66.71 hrs (+/- 27.12), p本研究旨在评估通过优化检测流程以缩短鉴定时长，使用基质辅助激光解吸电离飞行时间质谱（matrix assisted laser desorption ionization-time of flight, MALDI-TOF）能否缩短患者获得针对性抗菌治疗覆盖的时间。为此，我们开展了一项回顾性分析，纳入2016年1月至2017年12月期间住院革兰阴性菌血症患者的377份血培养样本，分别采用MALDI-TOF与标准鉴定方法维特克2号（VITEK 2）进行检测并对比。结果显示，MALDI-TOF可显著缩短从血培养采集至完成病原菌鉴定的时间间隔，且与启动更精准抗菌治疗的时间显著缩短相关，平均时长差值为16.37小时，95%置信区间为10.05至22.69（MALDI-TOF组平均时长：50.34小时±21.21，维特克2组：66.71小时±27.12，p