GUIDE-seq of NcCas9

NIAID Data Ecosystem2026-03-14 收录

CRISPR脱靶检测

基因编辑特异性

数据链接：

https://www.ncbi.nlm.nih.gov/sra/SRP398785 数据链接链接失效反馈

官方服务：

资源简介：

GUIDE-seq was constructed to assess the on-target and off-target frequencies of NcCas9 targeting Site1 and Site5

创建时间：

2022-09-24

Optimizing genome editing strategy by primer-extension-mediated sequencing

CRISPR脱靶检测

基因组优化

High specificity of e\ngineered nucleases ensures precise genome editing. Couple methods were developed to identify off-target sites of CRISPR/Cas9, but hardly any high-throughput sequencing method ca

NIAID Data Ecosystem60

Cas9 target DNA specificity

基因编辑特异性

CRISPR-Cas9靶点设计

Cas9 target DNA specificity

ChEBI2014-11-14 更新50

Genome-wide Profiling of Adenine Base Editor Specificity by EndoV-seq. Homo sapiens

碱基编辑器脱靶检测

基因编辑特异性

EndoV-seq utilizes Endonuclease V (EndoV) to nick the inosine (I)-containing DNA strand of genomic DNA that has been deaminated by ABE in vitro. The treated DNA is then whole-genome sequenced to ident

NIAID Data Ecosystem40

Number of GUIDE-seq datasets and identified digested sequences. The numbers in the parentheses include technical replicates.

CRISPR脱靶检测

基因编辑序列分析

Number of GUIDE-seq datasets and identified digested sequences. The numbers in the parentheses include technical replicates.

Figshare2025-07-18 更新60

Improving the sensitivity of in vivo CRISPR off-target detection through DNA repair inhibition. Homo sapiens and Mus musculus

CRISPR脱靶检测

基因编辑安全性

Improving the sensitivity of in vivo CRISPR off-target detection through DNA repair inhibition

NIAID Data Ecosystem50